Molecular characterization of ureaplasmas isolated from reproductive tract of goats and sheep from Brazil
نویسندگان
چکیده
Ovine/caprine ureaplasmas have not yet been assigned a species designation, but they have been classified into nine serotypes. Herein ureaplasmas were searched for in 120 samples of vulvo vaginal mucous from sheep and 98 samples from goats at 17 farms. In addition, semen samples were collected from 11 sheep and 23 goats. The recovered ureaplasma were from sheep and goats from animals without any reproductive disorder symptoms, but not all animals presented positive cultures. In sheep, 17 (68%) cultures of vulvovaginal mucous were positive for ureaplasma and 11 (27%) samples of semen presented positive cultures in animals with clinical signs of orchitis, balanoposthitis or low sperm motility. In goats four ureaplasma isolates were obtained from vulvovaginal mucus, but the semen samples were all negative. The isolates were submitted to Pulsed-field gel electrophoresis methodology and their 16S rRNA genes were sequenced. Fifty percent of ureaplasma recovered from sheep allowed for PFGE typing. Eleven isolates showed eight profiles genetically close to the bovine ureaplasmas. The 16S rRNA gene sequencing showed differences or similarities of isolates from sheep and goats, and the reference strains of bovine and human ureaplasma. Four clinical isolates from sheep were grouped separately. The studied ureaplasma isolates showed to be a diverse group of mollicutes.
منابع مشابه
Characterization, ribotyping and capsular PCR typing of Pasteurella multocida isolated from Iranian sheep and goats
Pasteurella multocida is considered to be an important cause of ovine pneumonia and results causes considerable economic losses in Iran. Ribotyping PCR (Ribo-PCR) was used for investigating the diversity of ovine and caprine P.multocida. A total of 120 swab tonsil and nasal samples were obtained from sheep and goats. They were analyzed by biochemical tests and Ribo-PCR 16S-23S ribosomal RNA gen...
متن کاملMolecular Characterization of Echinococcus granulosus Isolated from Sheep and Camel in Iran
متن کامل
Molecular typing of toxigenic Clostridum perfringens isolated from sheep in Iran
In this research a molecular method based on polymerase chain reaction for typing of Clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in Iran were determined. The PCR assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes A B, C and D. The field isolates ...
متن کاملIsolation, Molecular Characterization and Antibiotic Susceptibility Pattern of Pasteurella multocida Isolated from Cattle and Buffalo from Ahwaz, Iran
Pasteurella multocida is a gram-negative, opportunistic pathogen and the common inhabitant of the upper respiratory tract of domesticated and wild animal species. It is a causative agent of numerous economically important diseases worldwide, such as enzootic bronchopneumonia in ruminants and haemorrhagic septicemia in cattle and buffalo. The present study was undertaken to determine the prevale...
متن کاملPrevalence and molecular characterization of staphylococci isolated from sheep with subclinical mastitis in West-Azerbaijan province, Iran
This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of gly...
متن کامل